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NanoToxClass |
FP7 ERA-NET on
Nanosafety Safe Implementation of Innovative Nanoscience and Nanotechnology |
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| Work Package 5 |
| Biokinetics, Biodistribution, Cellular Uptake |
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aim of WP5 is to provide information on the dependency of biokinetics,
biodistribution, and cellular uptake of selected NMs on their
physico-chemical characteristics in supporting the establishment of
criteria for NM grouping. Biokinetics (accumulation characteristics)
and biodistribution of selected NMs of various sizes and surface
chemistries, in the various tissues of the rats (with emphasis on lung,
liver, kidney, and spleen), are important parameters for risk
assessment of NMs. The possibility to mimic the in vivo
cell-nanoparticle interaction under in vitro conditions is expected to
provide the possibility to correlate in vitro observations with in vivo
ones. Based on published literature and additional experiments, a
correlation between toxicological characteristics (inflammation,
oxidative stress, genotoxicity) observed under in vitro and in vivo
conditions by the same NM will be examined. Here we will assess the
interaction of nanoparticles with rat aveolar and macrophage
cell-lines, acting either on cell surface or intracellularlly following
their uptake. Attention will be devoted to the time-dependent
transformation of the NMs due to aggregation and agglomeration, as well
as to the possible uneven distribution of the NMs in the extracellular
medium because of possible precipitation. The mechanisms of the uptake
process will be addressed. WP5 tasks: Task 1 - Biokinetics, Biodistribution Task 2 - In vitro/ in vivo correlation Task 3 - Cellular Uptake, Uptake mechanisms |
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This project is part
of the SIINN ERA-NET and is funded under the ERA-NET scheme of the Seventh Framework Programme of the European Commission, Research Directorate - General, Grant Agreement No. 265799 |
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