FP7 ERA-NET on Nanosafety

Safe Implementation of Innovative
Nanoscience and Nanotechnology


Work Package 3

Toxicity Data and Testing

Inhalation is considered to be the most likely route of NM uptake for workers as for consumers. WP3 will look at the in vivo and in vitro toxicity of NMs using relevant models for inhalation. Our approach will be to consider data from literature and gathered from other projects for all relevant endpoints for identification of gaps and inconsistencies, which will then be filled in experimentally.
In vivo studies will be performed in rats to assess NMs biodistribution profile and toxicity effects. Lung alveolar macrophages are expected targets following NMs exposure. In vitro cytotoxicity studies will be conducted using a rat alveolar macrophage cell line to evaluate whether these cells are good predictors of in vivo toxicity. Established endpoints such as oxidative stress and inflammation will be evaluated both in vivo and in vitro. Various types of genetic damage will be investigated (e.g. single- and double-strand breaks, alkali sensitive sites, oxidative damage, chromosomal loss) in rat lung alveolar epithelial cells both in vivo and in vitro.

WP3 tasks:
Task 1 - In vivo instillation toxicity studies, in vitro cytotoxicity
Task 2 - Genotoxicity in vivo and in vitro
Task 3 - Oxidative stress in vivo and in vitro
Task 4 - Inflammation in vivo and in vitro

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This project is part of the SIINN ERA-NET and is funded under the ERA-NET scheme
of the Seventh Framework Programme of the European Commission,

Research Directorate - General, Grant Agreement No. 265799

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